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- The ability of the bacterial cell wall to hold onto the crystal violet dye after solvent treatment is the fundamental tenet of gram staining. Gram-positive microorganisms have more peptidoglycan; gram-positive bacteria lack an outer membrane but are encased in layers of peptidoglycan that are much thicker than those found in Gram-negative microorganisms. Gram-negative organisms contain more lipids than the opposite. A lipopolysaccharide-containing outer membrane encircles the peptidoglycan cell wall that surrounds gram-negative bacteria.
- Hey, Grammy! May I know what part of the bacterial cell plays an important role in determining whether it is Gram positive or Gram negative?
- How about you, Preppy. Why must a smear be heat-fixed before staining?
- Because the smear's microorganisms are destroyed by heat fixing, which also securely binds the smear to the slide and makes it easier for the sample to absorb stains.
- Also, Grammy. One more question. Which step is the most crucial in the Gram Staining procedure?
- The results of the Gram stain will be influenced by the thickness of the smear utilized. The decolorizing stage is very important in determining how the stain will turn out.
- Differential staining distinguishes between G+ and G- cells, whereas simple staining exposes only cell size. In a differential staining procedure, the primary stain imparts its color to all cells. Differential staining distinguishes between distinct bacterial species, whereas simple staining reflects only cell morphology.
- Ah, Simmy. What do you think the advantages of differential staining over simple staining technique?
- Oh, sure! Spore-forming bacteria endure extreme conditions of starvation, acidity, temperature, and dehydration by producing spores, which are resistant forms. Spores are unaffected by high temperatures and are able to germinate under favorable conditions, which may lead to complications.
- One more thing! Ah, Porry. May I know the importance of spore-forming bacteria in medical industry?
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