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DNA polymerase 1

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DNA polymerase 1
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  • That was a good presentation Ross Let us have Monica for her seminar on DNA Polymerase 1.
  • Thank You Sir. As Ross explained about DNA polymerase 2 and its important structure and functions, we will now learn about DNA Polymerase 1
  • DNA polymerase I is an enzyme involved in prokaryotic DNA elongation and repair. It has 3 activities: 5'-3' polymerase activity (synthesis), 5'-3' exonuclease activity and 3'-5' exonuclease activity
  • DNA polymerase I processes RNA primers during lagging-strand synthesis and fills small gaps during DNA repair reactions
  • A 5'→3' which is forward exonuclease activity mediating nick translation during DNA repair.A 5'→3' forward RNA-dependent DNA polymerase activity. Pol I operates on RNA templates with considerably lower efficiency (0.1–0.4%) than it does DNA templates, and this activity is probably of only limited biological significance
  • The polymerase reaction takes place only in the presence of an appropriate DNA template. To initiate this reaction, DNA polymerases require a primer with a free 3′-hydroxyl group already base-paired to the template. They cannot start from scratch by adding nucleotides to a free single-stranded DNA template.
  • Your presentation was very good. we understood everything
  • Exposure of DNA polymerase I to the protease subtilisin cleaves the molecule into a smaller fragment, which retains only the DNA polymerase and proofreading activities. Thank you guys . I have finished
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