That was a good presentation Ross Let us have Monica for her seminar on DNA Polymerase 1.
Thank You Sir. As Ross explained about DNA polymerase 2 and its important structure and functions, we will now learn about DNA Polymerase 1
DNA polymerase I is an enzyme involved in prokaryotic DNA elongation and repair. It has 3 activities: 5'-3' polymerase activity (synthesis), 5'-3' exonuclease activity and 3'-5' exonuclease activity
DNA polymerase I processes RNA primers during lagging-strand synthesis and fills small gaps during DNA repair reactions
A 5'→3' which is forward exonuclease activity mediating nick translation during DNA repair.A 5'→3' forward RNA-dependent DNA polymerase activity. Pol I operates on RNA templates with considerably lower efficiency (0.1–0.4%) than it does DNA templates, and this activity is probably of only limited biological significance
The polymerase reaction takes place only in the presence of an appropriate DNA template. To initiate this reaction, DNA polymerases require a primer with a free 3′-hydroxyl group already base-paired to the template. They cannot start from scratch by adding nucleotides to a free single-stranded DNA template.
Your presentation was very good. we understood everything
Exposure of DNA polymerase I to the protease subtilisin cleaves the molecule into a smaller fragment, which retains only the DNA polymerase and proofreading activities. Thank you guys . I have finished
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