Gram Staining
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- To find out the bacteria behind the infection, we have examined the samples under a microscope and gram stained them.
- Great, but before you tell me the bacteria identity, what's a gram stain?
- Well, when further determining the identity of bacteria, we examine the morphology and arrangement of the bacteria. Then we would do the gram staining process.
- When gram staining, we test the composition of the bacteria's cell wall. Gram negative bacteria appear pink and have a thin peptidoglycan layer. Gram positive bacteria appear purple and have a thick peptidoglycan layer. We do this because gross examination with the naked eye isn't enough to determine identity since colonies can have similar characteristics in appearance.
- So, after our examination and staining, the bacteria found in all patient samples were gram positive and had a cocci cluster morphology and arrangement.
- Our gram staining results match with the results of Staphylococcus aureus. So, we have finally identified the bacteria behind the infected patients.
- So now we will come up with a plan of treatment and prevention.
- Fantastic. Thank you so much for your help in solving this nosocomial nightmare.
- Great job. We solved the case.
- Yeah, time to write that plan now. 
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