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- Hans Christian Gram was the inventor of one of the most important systems used to identify bacteria. Gram Staining was a technique used to identify over 30,000 formally named species of bacteria. Gram staining is still used today in order to identify and classify bacteria: your doctor may use Gram staining in order to learn whether or not you have a bacterial infection and determine which species is causing it.
- Using crystal violet stained certain bacteria which would later be identified as gram-positive. Since these bacteria have a thick out layer of peptidoglycan, they were able to take up retain the crystal violet. Other bacteria which are known to be gram-negative do not have this retaining property and just end up looking red under the microscope.
- These differences resulted due to the composition of the bacterial cell wall. Some bacteria have a cell wall composed of peptidoglycan, which classified them as gram-positive. Due to the peptidoglycan wall, the cell was able to absorb the color of a stain and turn purple. Others bacterias that do not have a cell wall composed of peptidoglycan did not stain as well and appeared red.
- leave.
- Don't gotta be so "negative" all the time.
- Working alongside German pathologist and microbiologist Carl Friedlander, they created a system that was able to differentiate between different bacteria in a tissue lung tissue sample. Gram first dripped reagents onto the samples and discovered that there were visible differences in the coloring of bacteria.
- The colors look the same to me because of the sepia filter on this slide
- How intriguing, there is a clear color differentiation between the lung sample and the bacteria found within it, I believe we have found a new method of identifying bacteria!
- Grams technique back in 1884 differs from what we use today, but not by much. Gram experimented with staining pneumococci bacteria by modifying Ehrlich’s (is a reagent containing p-dimethylaminobenzaldehyde) alkaline aniline solutions. Gram stained his samples with aniline gentian violet, adding a solution of iodine and potassium for one to three minutes.
- Our team of scientists here at the Northwestern University in Boston, Massachusetts have been able to screen around 50,000 types of bacteria found in our soil for antibiotics.
- We do this in order to find antibiotics that would be able to kill bacteria such as MRSA and any bacteria that can cause multi-drug resistance teixobactin.
- Grams Staining technique is still employed all these years later as it has made indentifying teixobactin much easier as they belong to the gram-positive category and stain purple.
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